Regulatory properties of yeast hexokinase PII. Metal specificity, nucleotide specificity, and buffer effects.

Hexokinase specificity of some tumor tissue extracts.

The substrate specificity of hexokinase preparations from Ehrlich ascites cells, a spontaneous adenocarcinoma, Jensen sarcoma, and Walker carcinoma was studied with a number of sugars which differed in structure from glucose at one or more carbon atoms used as substrates. A marked variat ion in specificity was found. The preparations from Jensen sarcoma and Ehrlich ascites cells were relatively...

Immunological specificity of Staphylococal penicillinase

The hexose specificity of hexokinase in brain from various species.

the ketokinases from different sources differ so widely (liver <0-5 mm and rabbit muscle 0-2M; Hers, 1955). The constant calculated for the locustmuscle enzyme, 5-5 mm, is of the same order as that found for hexokinase from yeast, 1-7 mm, by Slein et al. (1950). We consider that locust muscle contains a single, non-specific hexokinase and that the enzyme is similar to that found in yeast and in...

Pyridine nucleotide specificity of barley nitrate reductase.

NADPH nitrate reductase activity in higher plants has been attributed to the presence of NAD(P)H bispecific nitrate reductases and to the presence of phosphatases capable of hydrolyzing NADPH to NADH. To determine which of these conditions exist in barley (Hordeum vulgare L. cv. Steptoe), we characterized the NADH and NADPH nitrate reductase activities in crude and affinity-chromatography-purif...

Substrate specificity of mammalian pyridine nucleotide transglycosidases.

Spectrophotometrical analysis of pyridine nucleotide transglycosidase in mammalian tissues indicated that the enzyme activity was observed to be distributed ubiquitously among the mammalian tissues analyzed, although the velocity ratio of transglycosidation/hydrolysis (vT/vH) and the partitioning of nicotinic acid against water differed with the pyridine nucleotide substrate and the mammalian s...